AAV pmSyn1-EBFP-Cre in Mouse (AAV Retrograde)
Addgene #51507-AAVrg
- Data Submitted By
- Henry Hallock and Keri Martinowich
- Submission Date
- June 19, 2019
- Publication Date
- June 19, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Henry Hallock
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
AAV pmSyn1-EBFP-Cre
Addgene #51507-AAVrg
Virus & Injection
Virus
- Virus Name
- AAV pmSyn1-EBFP-Cre
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 51507-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Recombinase
- Injection Titer / Dose
- 1.10E+13 GC/mL
- Injection Volume
- 600 nL
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Prelimbic area, NeuroNames ID: 1078
- Additional Details
- Injected into prelimbic sub-region of the medial prefrontal cortex.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 011029
- Age at Injection
- 3 months
- Time After Injection
- 4 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Ventral CA1 neurons with projections to the PrL.
- Target Cell Transduction
- The retro-Cre drove HA production very well in this mouse.
- Functional Testing
- We used a RiboTag mouse to drive HA protein expression with Cre, and this worked very well.
- Other Information
- No toxicity/inflammation.