pAAV.Syn.NES-jRGECO1a.WPRE.SV40 in Rat (AAV Retrograde)
Addgene #100854-AAVrg
- Data Submitted By
- Elizabeth Crummy and Susan Ferguson
- Submission Date
- June 21, 2019
- Publication Date
- June 21, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV.Syn.NES-jRGECO1a.WPRE.SV40
Addgene #100854-AAVrg -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
Addgene #51084-AAVrg
Virus & Injection
Virus 1
- Virus Name
- pAAV.Syn.NES-jRGECO1a.WPRE.SV40
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 100854-AAVrg
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 1.00E+13 GC/ml
- Injection Volume
- 1000 nL
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Nucleus accumbens, NeuroNames ID: 277
Virus 2
- Virus Name
- AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 51084-AAVrg
- Promoter
- Synapsin
- Injection Titer / Dose
- 9.00E+12 GC/ml
- Injection Volume
- 1000 nl
- Injection Rate
- 200 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Pons, NeuroNames ID: 547
- Additional Details
- Pyramidal tract (PT) cortical projection neurons projecting to the pons. Injected at same session as jRGECO.
Other Details
- Species
- Rat
- Strain or Cell Line
- Sprague Dawley
- Source
- Not provided
- Age at Injection
- ~2-3 months old
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry, fiber photometry recordings
- Assay & Results
- GCaMP PT expression, jRGECO IT expression.
- Target Cell Transduction
- Targeting intratelencephalic (IT) cortical neurons projecting to the nucleus accumbens. GCaMP PT expression, jRGECO IT expression.
- Functional Testing
- We have had issues with obtaining functional recordings during fiber photometry. Have had issues with jRGECO not expressing in some cohorts.
- Tips & Comments
- Maybe try to dilute the virus (particuarly GCaMP) and inject a lower volume.