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pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 in Mouse (AAV2)

Addgene #105545-AAV2

Data Submitted By
Hongbin Yang and Stephan Lammel
Submission Date
June 18, 2020
Publication Date
June 18, 2020 (modified May 16, 2023)
Abstract
Not provided
LDT (laterodorsal tegmental nucleus) neurons expressing retro-AAV-eGFP-cre. Cre: GFP (green); DAPI (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.
Caption: LDT (laterodorsal tegmental nucleus) neurons expressing retro-AAV-eGFP-cre. Cre: GFP (green); DAPI (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Addgene #105545-AAV2

Virus & Injection

Virus

Virus Name
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Serotype
AAV2
Source
Addgene
Catalog Number
105545-AAV2
Promoter
CMV
Cargo Type
Recombinase
Injection Titer / Dose
1.00E+12 GC/ml
Injection Volume
300 nl
Injection Rate
150 nl/min
Injector Material
Not provided
Injection Site / Route
Ventral tegmental area, NeuroNames ID: 521
Additional Details
Injection of the virus to VTA to map the inputs from LDTg; Midbrain: Tegmentum; Excitatory neurons in the brain stem area
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57BL/6J6J
Age at Injection
7 weeks
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Functional Testing
It worked as expected
Other Information
No toxicity or inflammation were observed via anatomic imaging within 3 weeks after virus infusion
Tips & Comments
The toxicity should be evaluated using in vivo recording in future. But so far, this virus works really well