pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 in Mouse (AAV2)

Addgene #105545-AAV2

Data Submitted By: Hongbin Yang and Stephan Lammel
Submission Date: June 18, 2020
Publication Date: June 18, 2020 (modified May 16, 2023)
Abstract: Not provided

**Caption:** LDT (laterodorsal tegmental nucleus) neurons expressing retro-AAV-eGFP-cre. Cre: GFP (green); DAPI (blue). A Zeiss AxioImager M2 upright widefield fluorescence/differential interference contrast microscope (5x objective) was used to obtain the image.

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vector Used: pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
#105545-AAV2

Virus & Injection

Virus

Virus Name: pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Serotype: AAV2
Catalog Number: 105545-AAV2
Promoter: CMV
Cargo Type: Recombinase
Injection Titer / Dose: 1.00E+12 GC/ml
Injection Volume: 300 nl
Injection Rate: 150 nl/min
Injector Material: Not provided
Injection Site / Route: Ventral tegmental area, NeuroNames ID: 521
Additional Details: Injection of the virus to VTA to map the inputs from LDTg; Midbrain: Tegmentum; Excitatory neurons in the brain stem area

View in Addgene Catalog

Other Details

Species: Mouse
Strain or Cell Line: C57BL/6J6J
Age at Injection: 7 weeks
Time After Injection: 3 weeks
Detection Method: Direct fluorescence
Functional Testing: It worked as expected
Other Information: No toxicity or inflammation were observed via anatomic imaging within 3 weeks after virus infusion
Tips & Comments: The toxicity should be evaluated using in vivo recording in future. But so far, this virus works really well