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pAAV-Syn-ChrimsonR-tdT in Mouse (AAV5)

Addgene #59171-AAV5

Data Submitted By
Jeff Conn and Max Joffe
Submission Date
July 03, 2019
Publication Date
July 03, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Chrimson-tdTomato in the basolateral amygdala

Any further use of this image requires permission from the lab who submitted the data. This scientist responded to a specific request for user data and was offered compensation for time and input.

Vectors Used
pAAV-Syn-ChrimsonR-tdT
#59171-AAV5
AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
#N/A

Virus & Injection

Virus 1

Virus Name
pAAV-Syn-ChrimsonR-tdT
Serotype
AAV5
Catalog Number
59171-AAV5
Promoter
Synapsin
Cargo Type
Optogenetics
Injection Titer / Dose
8.20E+12 GC/mL
Injection Volume
400 nL
Injection Rate
100 nL/min
Injector Material
Not Provided
Injection Site / Route
Basolateral nucleus of the amygdala, NeuroNames ID: 246
Additional Details
Basolateral amygdala pyramidal cells, and their projections to the prefrontal cortex. Basolateral amygdala projection neurons.
View in Addgene Catalog

Virus 2

Virus Name
AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
Serotype
N/A
Catalog Number
N/A
Promoter
CaMKII
Injection Titer / Dose
Unreported GC/mL
Injection Volume
400 nL
Injection Rate
100 nl/min
Injector Material
Not Provided
Injection Site / Route
Medial dorsal nucleus, NeuroNames ID : 312
Additional Details
AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Jackson Labs
Stock Number
Jax 000664
Age at Injection
4-6 weeks
Time After Injection
4 weeks
Detection Method
Direct fluorescence
Assay & Results
Confirmed red fluorescence in cell bodies in basolateral amygdala injection site and in axon terminals in prefrontal cortex. Then recorded excitatory postsynaptic currents in prefrontal cortex neurons evoked with light stimulation.
Functional Testing
Operated as expected.
Other Information
Worked well.
Tips & Comments
Worked as expected.