pAAV-hSyn-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #50475-AAV8

Acquisition done with a confocal microscope. One channel, for mCherry/RFP.

Caption: Acquisition done with a confocal microscope. One channel, for mCherry/RFP.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.26E+12 GC/ml
Injected Volume
400 nl
Injection Site
Brain parenchyma
Brain Region
Midbrain: Tegmentum
Injection Site Comments
Ventral tegmental area was targeted
View in Addgene Catalog

Subject

Species / Strain
Rat Wild-type Long Evans
Age at Injection
10 weeks

Data Collection

Time After Injection
Once injected, rats were trained to a task which, on average, requires a 4-week long training to become proficient. from then on, i.e. in expert rats, we started taking advantage of the dreadds expression.
Detection Method
Direct fluorescence
Expression Site
Ubiquitous, as expected