pAAV-CamKII-ArchT-GFP (PV2527) in Zebra finch (AAV1)
Addgene #99039-AAV1

488 Green = Anti GFP for labeling ArchT expression.  594 Red = DARPP32 Marker for Spiny Neurons  Zeiss 710 Inverted Confocal Microscope  20x Magnification  Tear down the middle is optogenetic probe placement after experiment completion.

Caption: 488 Green = Anti GFP for labeling ArchT expression. 594 Red = DARPP32 Marker for Spiny Neurons Zeiss 710 Inverted Confocal Microscope 20x Magnification Tear down the middle is optogenetic probe placement after experiment completion.

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
2.00E+12 GC/ml
Injected Volume
350 nl
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Targetting Medium Spiny Neurons In Area X
View in Addgene Catalog

Subject

Species / Strain
Zebra finch
Age at Injection
100 days

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
Area X, HVC
Genetic Tool Outcome
Light-induced suppression detected in anesthetized bird