pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV9)
Addgene #100842-AAV9

GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um.

Caption: GCaMP expressing neurons are visualized by anti-GFP staining (green). Coronal section. Blue, DAPI, 3V, third ventricle. Scale bar, 100 um.

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.90E+13 GC/ml
Injected Volume
400 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hypothalamus
Injection Site Comments
Oxytocinergic neurons in paraventricular hypothalamic nucleus
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
Jax mouse Stock No: 024234
Age at Injection
6 weeks old

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Oxytocinergic neurons in paraventricular hypothalamic nucleus
Genetic Tool Outcome
Yes