pAAV-CaMKIIa-hChR2(H134R)-EYFP in Mouse (AAV9)
Addgene #26969-AAV9

Unilateral injection of AAV9 into the anterior insular cortex. Imaged using an Olympus MVX10 microscope with an Olympus U-RFL-T power supply. Imaged at 0.63 X at 488 nm.

Caption: Unilateral injection of AAV9 into the anterior insular cortex. Imaged using an Olympus MVX10 microscope with an Olympus U-RFL-T power supply. Imaged at 0.63 X at 488 nm.

Image attribution: David L. Haggerty & Brady Atwood

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
1.00E+13 GC/ml
Injected Volume
50 nl
Injection Rate
25 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Anterior Insular Cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
Jackson Labs
Age at Injection
6 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Anterior Insular Cortex - CamkII positive neurons
Genetic Tool Outcome
Light was used to activate receptors - wireless optogenetics- Neurolux INC
Other Information
No inflammation or cell death
Tips & Comments
AAV9 has worked the best in our experience. The virus we use is undiluted, and has great expression. We leave the needle in the brain for 2 min post injection and retract slowly, which helps with limiting viral particles spreading from the injection site