pAAV-hSyn-DIO-mCherry in Rat (AAV8)
Addgene #50459-AAV8

Insular cortex neurons projecting to thalamic/habenular areas labeled using hSyn-DIO-mCherry and retrograde Cre viruses. Endogenous mCherry expression is depicted in red. Immuno-amplified mCherry (Chicken anti-mCherry, AlexaFluor488) expression in green shows finer morphology than endogenous expression.

Caption: Insular cortex neurons projecting to thalamic/habenular areas labeled using hSyn-DIO-mCherry and retrograde Cre viruses. Endogenous mCherry expression is depicted in red. Immuno-amplified mCherry (Chicken anti-mCherry, AlexaFluor488) expression in green shows finer morphology than endogenous expression.

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
500 nl
Injection Rate
5 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Insular Cortex (AP: 3. 0, ML: 3. 8, DV: 6. 0) projection neurons
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Secondary Virus
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)
Promoter
Syn
Injected Titer
1.20E+13 GC/ml
Injected Volume
200 nl
Injection Rate
1 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Epithalamus
View in Addgene Catalog

Subject

Species / Strain
Rat Long-Evans
Age at Injection
~3 months old

Data Collection

Time After Injection
8 weeks
Detection Method
Immunohistochemistry
Expression Site
Insular cortex neurons projecting to thalamic/habenula area
Genetic Tool Outcome
Yes, retrograde Cre mediated expression of the mCherry reporter construct was present in the injected region