pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV5)
Addgene #20298-AAV5

Right primary motor cortex expressing ChR2-YFP was shown. In situ hybridization was performed to enhance YFP signal (green).   Blue, DAPI.

Caption: Right primary motor cortex expressing ChR2-YFP was shown. In situ hybridization was performed to enhance YFP signal (green). Blue, DAPI.

Viruses & Injection

Primary Virus
Promoter
EF1a
Injected Titer
7.70E+12 GC/ml
Injected Volume
200 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Primary motor cortex
View in Addgene Catalog
Secondary Virus
pENN.AAV.hSyn.Cre.hGH (AAV9)
Promoter
Syn
Injected Titer
2.30E+13 GC/ml
Injected Volume
200 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Primary motor cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
6 weeks old

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Primary motor cortex
Genetic Tool Outcome
Yes