pENN.AAV.hSyn.Cre.WPRE.hGH in Rat (AAV Retrograde)
Addgene #105553-AAVrg

Prelimbic cortex neurons labeled by mouse anti-Cre antibody following injection of retrograde Cre virus into medio dorsal thalamic region

Caption: Prelimbic cortex neurons labeled by mouse anti-Cre antibody following injection of retrograde Cre virus into medio dorsal thalamic region

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.20E+13 GC/ml
Injected Volume
200 nl
Injection Rate
1 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Epithalamus
Injection Site Comments
Targeted neurons (hSyn promoter) in the Lateral Habenula
View in Addgene Catalog

Subject

Species / Strain
Rat Long-Evans
Age at Injection
~100 days old

Data Collection

Time After Injection
8-12 weeks
Detection Method
Immunohistochemistry
Expression Site
Neurons in the Lateral Habenula (injection site) and retrogradely labeled brain areas that project to the Lateral Habenula (eg, Lateral Hypothalamus)
Genetic Tool Outcome
The virus operated as expected. Retrograde labeling was detected via immunohistochemistry in region that project to the brain region targeted by the virus injection
Other Information
No oddities were observed in the studies using this virus