pZac2.1 gfaABC1D-lck-GCaMP6f in Mouse (AAV5)
Addgene #52924-AAV5

Acute cortical slice in V1 showing GCaMP+ astrocytes

Caption: Acute cortical slice in V1 showing GCaMP+ astrocytes

Image attribution: Michelle Cahill

Virus & Injection

Primary Virus
Promoter
GFAP
Injected Titer
4.92E+13 GC/ml
Injected Volume
30 nl/site, 8 sites per animal, 240 nl total
Injection Rate
3 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Targeted astrocytes in V1
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
P0–3

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Cortical astrocytes
Genetic Tool Outcome
Calcium dynamics looked as expected