Skip to main content

pAAV-GFAP-hM4D(Gi)-mCherry in Rat (AAV5)

Addgene #50479-AAV5

Data Submitted By
Zafar Iqbal and Ying Li
Submission Date
July 28, 2019
Publication Date
July 28, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: hm4Di (red) colabel with astrocytes (Green) and DAPi (blue)

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-GFAP-hM4D(Gi)-mCherry
Addgene #50479-AAV5

Virus & Injection

Virus

Virus Name
pAAV-GFAP-hM4D(Gi)-mCherry
Serotype
AAV5
Source
Addgene
Catalog Number
50479-AAV5
Promoter
GFAP
Cargo Type
Chemogenetics
Injection Titer / Dose
2.50E+12 GC/ml
Injection Volume
1 μl
Injection Rate
100 nl/min
Injector Material
Not Provided
Injection Site / Route
Hippocampus NeuroNames ID : 3157
Additional Details
Astrocytes; Forebrain: Hippocampus; HPC astrocytes
View in Addgene Catalog

Other Details

Species
Rat
Strain or Cell Line
Sprague Dawley
Source
Not provided
Age at Injection
250 to 300grams
Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Assay & Results
Not Provided
Functional Testing
CNO was used to activate hm4Di