pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #44362-AAV8

Left panel shows expression of ChAT and mCherry (reporter) in the same cell. Right panel shows mCherry positive axons in the medial prefrontal cortex

Caption: Left panel shows expression of ChAT and mCherry (reporter) in the same cell. Right panel shows mCherry positive axons in the medial prefrontal cortex

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
5.00E+12 GC/ml
Injected Volume
0.5 μl
Injection Rate
Bolus
Injection Site
Brain parenchyma
Brain Region
Nucleus basalis
Injection Site Comments
Cholinergic neurons in the nucleus basalis that express cre recombinase
View in Addgene Catalog

Subject

Species / Strain
Rat ChAT::Cre
Age at Injection
3 months

Data Collection

Time After Injection
4 weeks
Detection Method
Immunohistochemistry
Expression Site
Cholinergic neuron cell bodies and axons originating from nucleus basalis
Genetic Tool Outcome
The vector expressed the inhibitory DREADD hM4Di. Behavioral data suggested the inhibition worked
Other Information
Did not observe any non-specific damage
Tips & Comments
Only tested AAV8. Was recommended to work best. Actual inhibition produced by CNO or C21 injection should be examined. We have a protocol for this, but was running short on time, so could not implement it