pAAV-GFAP-hM3D(Gq)-mCherry in Mouse (AAV5)
Addgene #50478-AAV5

Zeiss Axioskop, 5x objective, red = mCherry, blue = DAPI

Caption: Zeiss Axioskop, 5x objective, red = mCherry, blue = DAPI

Image attribution: Sae Yokoyama

Virus & Injection

Primary Virus
Promoter
GFAP
Injected Titer
1.20E+13 GC/ml
Injected Volume
400-600 nl
Injection Rate
0. 5-1 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
V1 cortical astrocytes
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
2–5 months postnatal

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Cortical astrocytes
Genetic Tool Outcome
Yes, chemogenetic change in calcium in astrocytes