pAAV-GFAP-hM3D(Gq)-mCherry in Mouse (AAV5)
Addgene #50478-AAV5
- Data Submitted By
- Kira Poskanzer
- Submission Date
- July 25, 2019
- Publication Date
- July 25, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Image attribution: Sae Yokoyama
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pAAV-GFAP-hM3D(Gq)-mCherry
Addgene #50478-AAV5
Virus & Injection
Virus
- Virus Name
- pAAV-GFAP-hM3D(Gq)-mCherry
- Serotype
- AAV5
- Source
- Addgene
- Catalog Number
- 50478-AAV5
- Promoter
- GFAP
- Cargo Type
- Chemogenetics
- Injection Titer / Dose
- 1.20E+13 GC/ml
- Injection Volume
- 400-600 nl
- Injection Rate
- 30-60 nl/min
- Injector Material
- Not Provided
- Injection Site / Route
- Primary visual cortex, NeuroNames ID: 2102
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Not provided
- Age at Injection
- 2–5 months postnatal
- Time After Injection
- 3 weeks
- Detection Method
- Immunohistochemistry, Direct fluorescence
- Assay & Results
- V1 cortical astrocytes; Cortical astrocytes.
- Functional Testing
- Yes, chemogenetic change in calcium in astrocytes.