pAAV-hSyn-DIO-mCherry in Mouse (AAV8)
Addgene #50459-AAV8

blue: DAPI.  red: mCherry (no staining).  10x objective

Caption: blue: DAPI. red: mCherry (no staining). 10x objective

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.00E+12 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hypothalamus
Injection Site Comments
The suprachiasmatic nucleus (SCN) is targeted
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J with Drd1-Cre
Stock Number
Cited in https://doi.org/10.1002/dvg.20405
Age at Injection
10 weeks

Data Collection

Time After Injection
2 weeks
Detection Method
Direct fluorescence
Expression Site
This virus is hard to get into SCN. You can see it in the attached image. AAV1 might work better than this (AAV8)
Genetic Tool Outcome
Not determined, it is for control group
Tips & Comments
I will use AAV1