pAAV-hSyn-hM4D(Gi)-mCherry in Mouse (AAV2)
Addgene #50475-AAV2

Coronal mouse brain section  of  the MEC layer 2 long range projections in dentate gyrus labeling with DREADD receptor hM3D or hM4D was taken by Leica confocal microscope under 20X. Red channel represents mCherry, and blue color is DAPI.

Caption: Coronal mouse brain section of the MEC layer 2 long range projections in dentate gyrus labeling with DREADD receptor hM3D or hM4D was taken by Leica confocal microscope under 20X. Red channel represents mCherry, and blue color is DAPI.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.00E+12 GC/ml
Injected Volume
0.5 μl
Injection Site
Brain parenchyma
Brain Region
Medial entorhial cortex
Injection Site Comments
Medial entorhial cortex provides input to the dentate gyrus. Our goal is to target the perforate path fiber in dentate gyrus,
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
P1 to P3

Data Collection

Time After Injection
2 or 3 weeks
Detection Method
Direct fluorescence
Expression Site
The perforant path fiber in dentate gyrus
Genetic Tool Outcome
We used CON to active the hM3D and hM4d. We saw the c-fos expression was changed dramatically
Tips & Comments
The expression pattern looked the same for the excitatory DREADD (50474-AAV2)