pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Rat (AAV1)
Addgene #98928-AAV1

Apical dendrite of Prelimbic cortex projection neurons expressing AAV1.FLeX.sm-Flag. Immunolabeling was used to identify the following targets -- Flag: Red; cFos: Green; DAPI: Blue. A Zeiss 880 confocal microscope (20x mag,) was used to obtain this image.

Caption: Apical dendrite of Prelimbic cortex projection neurons expressing AAV1.FLeX.sm-Flag. Immunolabeling was used to identify the following targets -- Flag: Red; cFos: Green; DAPI: Blue. A Zeiss 880 confocal microscope (20x mag,) was used to obtain this image.

Viruses & Injection

Primary Virus
Promoter
CAG
Injected Titer
2.20E+13 GC/ml
Injected Volume
500 nl each in prelimibic and infralimbic cortices
Injection Rate
5 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injections were targeted at the prelimbic and Infralimbic cortex projection neurons
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Secondary Virus
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)
Promoter
Syn
Injected Titer
1.20E+13 GC/ml
Injected Volume
200 nl
Injection Rate
1 nl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Epithalamus
Injection Site Comments
Targeting neurons using a pan-neuronal hSyn promoter
View in Addgene Catalog

Subject

Species / Strain
Rat Long-Evans
Age at Injection
~100 days old

Data Collection

Time After Injection
~10 weeks
Detection Method
Immunohistochemistry
Expression Site
Prelimbic and Infralimibic projection neurons were targeted and labeled with the protein encoded by the virus used
Genetic Tool Outcome
Yes, the cells were labeled well with the Cre-dependent reporter virus
Other Information
No toxicity or inflammation were observed
Tips & Comments
At the present time, I have no additional comments. The virus works as described and expected based on the initial report (Viswanathan et al, 2015)