pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 in Rat (AAV1)
Addgene #98928-AAV1
- Data Submitted By
- Todd Nentwig and L. Judson Chandler
- Submission Date
- July 26, 2019
- Publication Date
- July 26, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vectors Used
-
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Addgene #98928-AAV1 -
pENN.AAV.hSyn.Cre.WPRE.hGH
Addgene #105553-AAVrg
Virus & Injection
Virus 1
- Virus Name
- pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 98928-AAV1
- Promoter
- CAG
- Cargo Type
- Control
- Injection Titer / Dose
- 2.20E+13 GC/mL
- Injection Volume
- 500 nL each in prelimibic and infralimbic cortices
- Injection Rate
- 250 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Prelimbic area, NeuroNames ID: 1078
- Additional Details
- Injections were targeted at the prelimbic and Infralimbic cortex projection neurons.
Virus 2
- Virus Name
- pENN.AAV.hSyn.Cre.WPRE.hGH
- Serotype
- AAV Retrograde
- Source
- Addgene
- Catalog Number
- 105553-AAVrg
- Promoter
- Synapsin
- Injection Titer / Dose
- 1.20E+13 GC/mL
- Injection Volume
- 200 nL
- Injection Rate
- 60 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Epithalamus, NeuroNames ID: 292
- Additional Details
- Targeting neurons using a pan-neuronal hSyn promoter.
Other Details
- Species
- Rat
- Strain or Cell Line
- Long-Evans
- Source
- Not provided
- Age at Injection
- ~100 days old
- Time After Injection
- ~10 weeks
- Detection Method
- Immunohistochemistry
- Assay & Results
- Prelimbic and Infralimibic projection neurons were targeted and labeled with the protein encoded by the virus used.
- Target Cell Transduction
- Yes, the cells were labeled well with the Cre-dependent reporter virus.
- Other Information
- No toxicity or inflammation were observed.
- Tips & Comments
- At the present time, I have no additional comments. The virus works as described and expected based on the initial report (Viswanathan et al, 2015).