AAV.CamKII.GCaMP6s.WPRE.SV40 in Mouse (AAV9)
Addgene #107790-AAV9

Images in two-photon microscope with galvometric scanning (~0.5 Hz), field of view 0.5 x 0.5 mm, super 20x objective with NA = 0.8, green channel (alkali PMT).

Caption: Images in two-photon microscope with galvometric scanning (~0.5 Hz), field of view 0.5 x 0.5 mm, super 20x objective with NA = 0.8, green channel (alkali PMT).

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
2.00E+11 GC/ml
Injected Volume
500 nl
Injection Rate
0. 03 ul/min to prevent backflow
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
I was targeting all excitatory cells in the barrel cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
6-10 weeks

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry, Direct fluorescence
Expression Site
Expression was local to the barrel cortex. Assumed excitatory cells, but we did not attempt to verify co-localization between the virus expression and CamKII as these experiments were largely used for testing
Other Information
Injection was at multiple sites (-300 um, -450 um, and -600 um) below the dura to prevent backflow but also spread the virus more equally