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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV9)

Addgene #100842-AAV9

Data Submitted By
Yair Deitcher and Michael London
Submission Date
July 20, 2019
Publication Date
July 20, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: GCaMP6s in L5 pyramidal cells

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Addgene #100842-AAV9

Virus & Injection

Virus

Virus Name
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40
Serotype
AAV9
Source
Addgene
Catalog Number
100842-AAV9
Promoter
CAG
Cargo Type
Biosensor
Injection Titer / Dose
6.56E+12 GC/ml
Injection Volume
1000 nl
Injection Rate
2 μl/sec
Injector Material
Not Provided
Injection Site / Route
Whisker sensory area (Barrel Cortex), NeuroNames ID: 2703
Additional Details
Barrel cortex; Forebrain: Cerebral cortex (neocortex); L5 pyramidal cells
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
RBP4
Source
Not provided
Age at Injection
2 month
Time After Injection
2 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Functional Testing
Yes, worked well
Other Information
When not diluted was toxic