pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV9)
Addgene #100842-AAV9

GCaMP6s in L5 pyramidal cells

Caption: GCaMP6s in L5 pyramidal cells

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
6.56E+12 GC/ml
Injected Volume
1000 nl
Injection Rate
2 μl/sec
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Barrel cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse RBP4 mice
Age at Injection
2 month

Data Collection

Time After Injection
2 weeks
Detection Method
Direct fluorescence
Expression Site
L5 pyramidal cells
Genetic Tool Outcome
Yes, worked well
Other Information
When not diluted was toxic