pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8

Dreadd (hm3d-mcherry) in red (610 emission), in cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line, epifluorescence microscope, 4x objective

Caption: Dreadd (hm3d-mcherry) in red (610 emission), in cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line, epifluorescence microscope, 4x objective

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
3.00E+12 GC/ml
Injected Volume
60 nl
Injection Rate
0. 3 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hypothalamus
Injection Site Comments
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
2 months

Data Collection

Time After Injection
8 weeks
Detection Method
Direct fluorescence
Expression Site
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
Genetic Tool Outcome
The DREADD receptor worked as expected upon treatment with systemic CNO injection