pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA in Mouse (AAV9)
Addgene #20298-AAV9

cholecystokinin (cck) cells in the hypothalamus of a cck-cre  expressing cre-dependent chr2-gfp (green, 510 emission channel), 4x objective, epifluorescence microscope

Caption: cholecystokinin (cck) cells in the hypothalamus of a cck-cre expressing cre-dependent chr2-gfp (green, 510 emission channel), 4x objective, epifluorescence microscope

Virus & Injection

Primary Virus
Promoter
EF1a
Injected Titer
2.00E+12 GC/ml
Injected Volume
60 nl
Injection Rate
0. 3 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hypothalamus
Injection Site Comments
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
2 months

Data Collection

Time After Injection
7 weeks
Detection Method
Direct fluorescence
Expression Site
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
Genetic Tool Outcome
Chr2 was activated with blue light and the behavior worked