AAV-FLEX-Arch-GFP in Mouse (AAV9)
Addgene #22222-AAV9

cck+ cells in hypothalamus,  arch-gfp (green) is 510 nm emission channel, 5x objective

Caption: cck+ cells in hypothalamus, arch-gfp (green) is 510 nm emission channel, 5x objective

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
2.00E+12 GC/ml
Injected Volume
40 nl
Injection Rate
0. 3 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hypothalamus
Injection Site Comments
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
2 months

Data Collection

Time After Injection
8 weeks
Detection Method
Direct fluorescence
Expression Site
Cholecystokinin (cck) cells in the hypothalamus of a cck-cre mouse line
Genetic Tool Outcome
Green light was used to activate arch, and the behavior worked