pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg

Motor cortex, 200u depth, red channel, 2p microscope 20X

Caption: Motor cortex, 200u depth, red channel, 2p microscope 20X

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.20E+13 GC/ml
Injected Volume
450 nl
Injection Rate
50 μl/min
Injection Site
Cerebrospinal fluid (CSF): Intracerebroventricular (ICV)
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Motor Cortex (contralateral of area of imaging). Targeting IT neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
Stock Number
JAX: 024742
Age at Injection
p54

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
IT neurons in the contralateral motor cortex
Other Information
No toxicity no inflammation
Tips & Comments
Nice expression in the contralateral as well as in the ipsilateral motor cortex