pAAV-CAG-tdTomato (codon diversified) in Mouse (AAV Retrograde)
Addgene #59462-AAVrg

Cortex 500u depth. Red channel, 2photon microscope, 20X objective, zoom=2,

Caption: Cortex 500u depth. Red channel, 2photon microscope, 20X objective, zoom=2,

Motor cortex 600u depth, red channel, 2p microscope, 20X objective

Caption: Motor cortex 600u depth, red channel, 2p microscope, 20X objective

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
1.20E+13 GC/ml
Injected Volume
450 nl
Injection Rate
50 μl/min
Injection Site
Cerebrospinal fluid (CSF): Intracerebroventricular (ICV)
Brain Region
Hindbrain: Pons
Injection Site Comments
AP -4. 26, ML 0. 6, DV 4. 6
View in Addgene Catalog

Subject

Species / Strain
Mouse B6;DBA-Tg(tetO-GCaMP6s)2Niell/J
Stock Number
JAX: 024742
Age at Injection
p45

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
PT neurons in the motor cortex
Other Information
I injected in the pons and looked for expression in the motor cortex. Not all the animals had somas in the field of view, but I could see axons in most of them
Tips & Comments
It requires multiple animals to end up with an animal with sufficient expression. 60% of success rate that could improve with multiple injections instead of only one