pAAV.hSynapsin.SF-iGluSnFR.A184S in Mouse (AAV1)
Addgene #106174-AAV1

blue (DAPI) green (FITC) . optic fiber track visualized above injection site

Caption: blue (DAPI) green (FITC) . optic fiber track visualized above injection site

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
50 nl
Injection Rate
10 nl/min
Injection Site
Brain parenchyma
Brain Region
Ventral striatum
Injection Site Comments
Wild type, all cells. Subregion targetted: nucleus accumbens medial shell
View in Addgene Catalog

Subject

Species / Strain
Mouse wild type swiss webster
Age at Injection
8 months

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
Nucleus accumbens medial shell with minor spillover to ventral lateral septum (no cell specificity)
Genetic Tool Outcome
Good signal detected using fiber photometry
Tips & Comments
Tends to spread a bit, would inject less than GCamp, for example