pAAV-Syn-ChrimsonR-tdT in Mouse (AAV9)
Addgene #59171-AAV9

Red channel: Chrimson expression - tdTomato  Green channel: cFos expression - Alexa488  Leica upright fluorescence microscope  10x objective

Caption: Red channel: Chrimson expression - tdTomato Green channel: cFos expression - Alexa488 Leica upright fluorescence microscope 10x objective

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.70E+12 GC/ml
Injected Volume
100 μl
Injection Rate
Bolus injection
Injection Site
Intravenous (retro-orbital or tail vein)
Injection Site Comments
Systemic injection through the tail vein. The blood-brain barrier was locally and non-invasively disrupted using focused ultrasound. We intended to transfect neurons within the cortex and hippocampus
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
3-6 months

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Cortical and hippocampal neurons
Genetic Tool Outcome
It operated as expected. Red light (635nm) was used to activate the neural network. Activation was detected through cFos staining post-mortem
Other Information
No toxicity or inflammation due to the AAV expression was observed
Tips & Comments
PAAV-Syn-ChrimsonR-tdT (AAV9) employs the Syn promoter. Other promoters targeting more specific neuronal types would be welcome