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pAAV-Syn-ChrimsonR-tdT in Mouse (AAV9)

Addgene #59171-AAV9

Data Submitted By
Antonios Pouliopoulos and Elisa Konofagou
Submission Date
July 11, 2019
Publication Date
July 11, 2019 (modified May 16, 2023)
Abstract
Not Provided
Caption: Red channel: Chrimson expression - tdTomato Green channel: cFos expression - Alexa488 Leica upright fluorescence microscope 10x objective

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV-Syn-ChrimsonR-tdT
Addgene #59171-AAV9

Virus & Injection

Virus

Virus Name
pAAV-Syn-ChrimsonR-tdT
Serotype
AAV9
Source
Addgene
Catalog Number
59171-AAV9
Promoter
Synapsin
Cargo Type
Optogenetics
Injection Titer / Dose
1.70E+12 GC/ml
Injection Volume
100 μl
Injection Rate
N/A
Injector Material
N/A
Injection Site / Route
Intravenous, tail vein
Additional Details
Systemic injection through the tail vein. The blood-brain barrier was locally and non-invasively disrupted using focused ultrasound. We intended to transfect neurons within the cortex and hippocampus; Cortical and hippocampal neurons
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
C57/BL6J
Source
Not provided
Age at Injection
3-6 months
Time After Injection
3 weeks
Detection Method
Direct fluorescence
Assay & Results
Not Provided
Functional Testing
It operated as expected. Red light (635nm) was used to activate the neural network. Activation was detected through cFos staining post-mortem
Other Information
No toxicity or inflammation due to the AAV expression was observed
Tips & Comments
PAAV-Syn-ChrimsonR-tdT (AAV9) employs the Syn promoter. Other promoters targeting more specific neuronal types would be welcome