pAAV-hSyn-DIO-mCherry in Mouse (AAV5)
Addgene #50459-AAV5

Imaged in the ventral hippocampus with a 10x objective on a confocal microscope, laser wavelength of 555 nm.

Caption: Imaged in the ventral hippocampus with a 10x objective on a confocal microscope, laser wavelength of 555 nm.

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.10E+12 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Injected into the ventral hippocampus to target CA1 cells projecting to the medial prefrontal cortex
View in Addgene Catalog
Secondary Virus
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)
Promoter
Syn
Injected Titer
1.20E+13 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Prelimbic subregion of the medial PFC
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
Mutant mice with disruption of BDNF from promoter IV (bred in-house: Maynard et al., https://www.nature.com/articles/npp2015349)
Age at Injection
3 months

Data Collection

Time After Injection
6 weeks
Detection Method
Direct fluorescence
Expression Site
Ventral hippocampal CA1 pyramidal neurons projecting to the mPFC
Genetic Tool Outcome
No tool was used
Other Information
No toxicity/inflammation
Tips & Comments
I would use this again for this experiment. Worked well