pGP-AAV-syn-jGCaMP7f-WPRE in Mouse (AAV1)
Addgene #104488-AAV1

GFP fluorescence imaged in mPFC with a 20x objective on a confocal microscope

Caption: GFP fluorescence imaged in mPFC with a 20x objective on a confocal microscope

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.20E+13 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injected into the prelimbic subregion of the medial prefrontal cortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
3 months

Data Collection

Time After Injection
8 weeks
Detection Method
Direct fluorescence
Expression Site
Neurons in the prelimbic cortex (pan-neuronal)
Genetic Tool Outcome
No tools used
Other Information
No toxicity/inflammation
Tips & Comments
The GCaMP7f worked well, but there was not a detectable difference between GCaMP7f and GCaMP6f for in vivo imaging in mPFC