pGP-AAV-syn-jGCaMP7f-WPRE in Mouse (AAV1)
Addgene #104488-AAV1
- Data Submitted By
- Henry Hallock and Keri Martinowich
- Submission Date
- July 11, 2019
- Publication Date
- July 11, 2019 (modified May 16, 2023)
- Abstract
- Not Provided
Any further use of this image requires permission from the lab who submitted the data.
- Vector Used
-
pGP-AAV-syn-jGCaMP7f-WPRE
Addgene #104488-AAV1
Virus & Injection
Virus
- Virus Name
- pGP-AAV-syn-jGCaMP7f-WPRE
- Serotype
- AAV1
- Source
- Addgene
- Catalog Number
- 104488-AAV1
- Promoter
- Synapsin
- Cargo Type
- Biosensor
- Injection Titer / Dose
- 2.20E+13 GC/mL
- Injection Volume
- 600 nL
- Injection Rate
- 200 nL/min
- Injector Material
- Not Provided
- Injection Site / Route
- Prelimbic area, NeuroNames ID: 1078
- Additional Details
- Injected into the prelimbic subregion of the medial prefrontal cortex.
Other Details
- Species
- Mouse
- Strain or Cell Line
- C57/BL6J
- Source
- Jackson Labs
- Stock Number
- JAX: 000664
- Age at Injection
- 3 months
- Time After Injection
- 8 weeks
- Detection Method
- Direct fluorescence
- Assay & Results
- Neurons in the prelimbic cortex (pan-neuronal).
- Functional Testing
- No tools used.
- Other Information
- No toxicity/inflammation.
- Tips & Comments
- The GCaMP7f worked well, but there was not a detectable difference between GCaMP7f and GCaMP6f for in vivo imaging in mPFC.