pAAV.hSyn1.Twitch2B.WPRE.SV40 in Mouse (AAV1)
Addgene #100040-AAV1

live slice on Nikon A1RMP+ Multiphoton with 25x objective, CFP/YFP filter was used to detect Twitch fluorescence (blue and green respectively in the image)

Caption: live slice on Nikon A1RMP+ Multiphoton with 25x objective, CFP/YFP filter was used to detect Twitch fluorescence (blue and green respectively in the image)

Image attribution: Katherine Sadleir

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+10 GC/ml
Injected Volume
4 μl (2 μl per hemisphere)
Injection Rate
Done by hand
Injection Site
Cerebrospinal fluid (CSF): Intracerebroventricular (ICV)
Brain Region
We target the ventricles
Injection Site Comments
Neurons were targeted
View in Addgene Catalog

Subject

Species / Strain
Mouse B6SJL F1 hybrid
Stock Number
100012
Age at Injection
P0 (first 24 hrs after birth)

Data Collection

Time After Injection
1 month to 6 months
Detection Method
Direct fluorescence
Expression Site
Neurons of the forebrain, cortex, hippocampus some midbrain as well
Genetic Tool Outcome
This is a ratiometric calcium sensor- different cells showed different ratios, responded to calcium changes
Other Information
No signs of toxicity or damage, expression lasted lifespan of animal (up to 16 months at least)