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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV5)

Addgene #100845-AAV5

Data Submitted By
Heidi Meyer and Francis Lee
Submission Date
July 10, 2019
Publication Date
July 10, 2019 (modified May 16, 2023)
Abstract
Not Provided
This image was taken at 10x and is the same brain slice as the previous image (provided to show spread of expression with 190 nl volume).
Caption: This image was taken at 10x and is the same brain slice as the previous image (provided to show spread of expression with 190 nl volume).
Image was taken at 10x magnification using a Nikon eclipse fluorescent microscope. Green cells indicate the presence of the Green Fluorescent Protein variant in the GCaMP molecule.
Caption: Image was taken at 10x magnification using a Nikon eclipse fluorescent microscope. Green cells indicate the presence of the Green Fluorescent Protein variant in the GCaMP molecule.

Any further use of this image requires permission from the lab who submitted the data.

Vector Used
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40
Addgene #100845-AAV5

Virus & Injection

Virus

Virus Name
pAAV.Syn.Flex.GCaMP6s.WPRE.SV40
Serotype
AAV5
Source
Addgene
Catalog Number
100845-AAV5
Promoter
Synapsin
Cargo Type
Biosensor
Injection Titer / Dose
1.10E+13 GC/mL
Injection Volume
190 nL
Injection Rate
50 nL/min
Injector Material
Not Provided
Injection Site / Route
Prelimbic area, NeuroNames ID: 1078
Additional Details
Prelimbic subregion of the prefrontal cortex. Targeting parvalbumin positive interneurons.
View in Addgene Catalog

Other Details

Species
Mouse
Strain or Cell Line
B6.129P2-Pvalbtm1(cre)Arbr/J obtained from Jackson Labs. Also known as B6 PVcre.
Source
Jackson Labs
Stock Number
Jax: 017320
Age at Injection
postnatal day 21-23
Time After Injection
2 weeks
Detection Method
Direct fluorescence
Assay & Results
Expression was largely localized to the prelimbic cortex, with some expression also observed in the medial orbitofrontal cortex and infralimbic cortex. Expression was limited to neurons expressing parvalbumin.
Functional Testing
The tool operated as expected. A 460 nm light was used to excite the GCaMP, and emitted fluorescence was recorded (fiber photometry).
Other Information
No toxicity or inflammation