pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 in Mouse (AAV5)
Addgene #100845-AAV5

Image was taken at 10x magnification using a Nikon eclipse fluorescent microscope. Green cells indicate the presence of the Green Fluorescent Protein variant in the GCaMP molecule.

Caption: Image was taken at 10x magnification using a Nikon eclipse fluorescent microscope. Green cells indicate the presence of the Green Fluorescent Protein variant in the GCaMP molecule.

This image was taken at 10x and is the same brain slice as the previous image (provided to show spread of expression with 190 nl volume)

Caption: This image was taken at 10x and is the same brain slice as the previous image (provided to show spread of expression with 190 nl volume)

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.10E+13 GC/ml
Injected Volume
190 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Prelimbic subregion of the prefrontal cortex. Targeting parvalbumin positive interneurons
View in Addgene Catalog

Subject

Species / Strain
Mouse B6.129P2-Pvalbtm1(cre)Arbr/J obtained from Jackson Labs. Also known as B6 PVcre.
Stock Number
Jax mouse Stock No: 017320
Age at Injection
postnatal day 21-23

Data Collection

Time After Injection
2 weeks
Detection Method
Direct fluorescence
Expression Site
Expression was largely localized to the prelimbic cortex, with some expression also observed in the medial orbitofrontal cortex and infralimbic cortex. Expression was limited to neurons expressing parvalbumin
Genetic Tool Outcome
The tool operated as expected. A 460 nm light was used to excite the GCaMP, and emitted fluorescence was recorded (fiber photometry)
Other Information
No toxicity or inflammation