pENN.AAV.hSyn.Cre.WPRE.hGH in Mouse (AAV1)
Addgene #105553-AAV1

in vivo two-photon image. Stitched from individual images that traced a single neuron's soma and its dendrites for ~100 microns as labeled by the combination of sparse Cre expression and Cre-dependent GCaMP6s

Caption: in vivo two-photon image. Stitched from individual images that traced a single neuron's soma and its dendrites for ~100 microns as labeled by the combination of sparse Cre expression and Cre-dependent GCaMP6s

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+08 GC/ml
Injected Volume
200 nl
Injection Rate
40 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse Cell type: any neuron
View in Addgene Catalog
Secondary Virus
pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
200 nl
Injection Rate
40 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Cell type: any neuron type
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
8-20 weeks

Data Collection

Time After Injection
5 weeks
Detection Method
Direct fluorescence
Expression Site
Dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Neuronal cell class
Other Information
This experiment targeted very sparse expression of GCaMP6s by combining Cre-dependent GCaMP6s expression with injection of very low titer of virus carrying Cre recombinase