pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 in Mouse (AAV1)
Addgene #100834-AAV1

in vivo two-photon imaging. 920 nm for excitation, 525 nm for emission

Caption: in vivo two-photon imaging. 920 nm for excitation, 525 nm for emission

Virus & Injection

Primary Virus
Promoter
CaMKIIa
Injected Titer
1.00E+12 GC/ml
Injected Volume
73.6 nl
Injection Rate
40 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse. Cell type: CaMKII-expressing excitatory cortical neurons
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
8-20 weeks

Data Collection

Time After Injection
5 weeks
Detection Method
Direct fluorescence
Expression Site
Injection site: dorsal anterior cingulate cortex/medial secondary motor cortex of mouse