pAAV.Syn.GCaMP6s.WPRE.SV40 in Mouse (AAV1)
Addgene #100843-AAV1

Image taken at 10x on a Nikon eclipse fluorescent microscope. Green cells reflect the presence of a GFP (green fluorescent protein variant) attached to the GCaMP molecule.

Caption: Image taken at 10x on a Nikon eclipse fluorescent microscope. Green cells reflect the presence of a GFP (green fluorescent protein variant) attached to the GCaMP molecule.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.50E+13 GC/ml
Injected Volume
200 nl
Injection Rate
50 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Targeting all neurons in the ventral subregion of the hippocampus
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
postnatal day 70-90 (adult)

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence
Expression Site
Expression was largely localized to the ventral hippocampus. In some cases, expression was also seen in dentate gyrus or entorhinal cortex. Expression was most notable in pyramidal neurons
Genetic Tool Outcome
The tool operated as expected. The virus expressed a calcium binding protein bound to a fluorescent reporter (GCaMP). Fluorescence was recorded using fiber photometry, following an emission of 470nm via an optical implant to excite the GCaMP
Other Information
I did not see toxicity or inflammation
Tips & Comments
No comments