pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Rat (AAV5)
Addgene #100842-AAV5

Blue - DAPI  Red  - TH Cre  Green - GFP    objective: 10X  microscope:  Zeiss 700 confocal

Caption: Blue - DAPI Red - TH Cre Green - GFP objective: 10X microscope: Zeiss 700 confocal

Image attribution: Jasmin Multani

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
3.20E+13 GC/ml
Injected Volume
two 1 μl injections
Injection Rate
75 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Noradrenergic cells of the locus coeruleus
View in Addgene Catalog

Subject

Species / Strain
Rat LE-Tg(TH-Cre)3.1Deis
Stock Number
RRRC#: 659
Age at Injection
3-4 months

Data Collection

Time After Injection
Tested 1 week to 8 weeks after injection
Detection Method
Immunohistochemistry, fiber photometry
Expression Site
Limited expression in noradrenergic cells of the locus coeruleus
Genetic Tool Outcome
I was able to get somewhat reliable fiber photometry signal
Other Information
No
Tips & Comments
I tried multiple capsids and promoters and found that this combination is the best