pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 in Rat (AAV9)
Addgene #100842-AAV9

Blue - DAPI  Red  - TH Cre  Green - GFP    objective: 20X  microscope:  Zeiss 700 confocal

Caption: Blue - DAPI Red - TH Cre Green - GFP objective: 20X microscope: Zeiss 700 confocal

Image attribution: Jasmin Multani

Virus & Injection

Primary Virus
Promoter
CAG
Injected Titer
5.20E+13 GC/ml
Injected Volume
two 1 μl injections
Injection Rate
75 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Noradrenergic cells of the locus coeruleus
View in Addgene Catalog

Subject

Species / Strain
Rat LE-Tg(TH-Cre)3.1Deis
Stock Number
RRRC#: 659
Age at Injection
3-4 months

Data Collection

Time After Injection
Tested 1 week to 8 weeks after injection
Detection Method
Immunohistochemistry, fiber photometry
Expression Site
Very limited expression in noradrenergic cells of locus coeruleus
Genetic Tool Outcome
Weak/unreliable signal on fiber photometry
Other Information
Yes - I experienced toxicity in the sense that several animals developed seizures at the higher titers (undiluted or diluted 1:5), but I would get no GCaMP signal at lower titers
Tips & Comments
I tested multiple capsids and promoters, and have found that AAV5-CAG works best