AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato in Rat (AAV1)
Addgene #51082-AAV1

Blue - DAPI. Magenta  - TH Cre. Green - GFP. Red - dTomato. objective: 10X. microscope: Zeiss 700 confocal.

Caption: Blue - DAPI. Magenta - TH Cre. Green - GFP. Red - dTomato. objective: 10X. microscope: Zeiss 700 confocal.

Image attribution: Jasmin Multani

Virus & Injection

Primary Virus
Promoter
EF1a
Injected Titer
1.30E+13 GC/ml
Injected Volume
two 1 μl injections
Injection Rate
75 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Noradrenergic cells of the locus coeruleus
View in Addgene Catalog

Subject

Species / Strain
Rat LE-Tg(TH-Cre)3.1Deis
Stock Number
RRRC#: 659
Age at Injection
3-4 months

Data Collection

Time After Injection
Checked with fiber photometry from 1 to 5 weeks after injection
Detection Method
Immunohistochemistry
Expression Site
No successful expression
Genetic Tool Outcome
Attempted to use fiber photometry to detect -- no signal
Other Information
No toxicity, but saw no expression
Tips & Comments
I tested multiple caspids and promoters -- AAV1 and AAV9 don't seem to express well in rat locus coeruleus, but AAV5 does okay. CAG is required for expression