pAAV-Ef1a-DIO ChETA-EYFP in Rat (AAV5)
Addgene #26968-AAV5

Blue - DAPI. Red  - TH Cre. Green - YFP. objective: 10X. microscope: Zeiss 700 confocal.

Caption: Blue - DAPI. Red - TH Cre. Green - YFP. objective: 10X. microscope: Zeiss 700 confocal.

Image attribution: Jasmin Multani

Virus & Injection

Primary Virus
Promoter
EF1a
Injected Titer
1.90E+13 GC/ml
Injected Volume
two 1 μl injections
Injection Rate
75 nl/min
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Noradrenergic cells of the locus coeruleus
View in Addgene Catalog

Subject

Species / Strain
Rat LE-Tg(TH-Cre)3.1Deis
Stock Number
RRRC#: 659
Age at Injection
3-4 months

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Expression Site
Noradrenergic cells of the locus coeruleus
Genetic Tool Outcome
Yes; in one animal cortical desynchronization was confirmed upon light stimulation Parameters: 500 ms, 10 Hz, 1-3 mW, 10 ms pulse duration of blue light stimulation