pAAV-Ef1a-DIO EYFP in Rat (AAV5)
Addgene #27056-AAV5

Blue - DAPI  Red  - TH Cre  Green - YFP    objective: 10X  microscope: Zeiss 700 Confocal

Caption: Blue - DAPI Red - TH Cre Green - YFP objective: 10X microscope: Zeiss 700 Confocal

Image attribution: Jasmin Multani

Virus & Injection

Primary Virus
Promoter
EF1a
Injected Titer
3.30E+13 GC/ml
Injected Volume
two 1 μl injections
Injection Rate
75 nl/sec
Injection Site
Brain parenchyma
Brain Region
Hindbrain: Pons
Injection Site Comments
Noradrenergic cells of the locus coeruleus
View in Addgene Catalog

Subject

Species / Strain
Rat LE-Tg(TH-Cre)3.1Deis
Stock Number
RRRC#: 00659
Age at Injection
3-4 months

Data Collection

Time After Injection
2 weeks
Detection Method
Immunohistochemistry
Expression Site
Noradrenergic cells of the locus coeruleus
Tips & Comments
AAV5 works well for this cell type