pAAV-hSyn-EGFP in Rat (AAV2)
Addgene #50465-AAV2

Endogenous EGFP reporter in green. Cell body expression in medial septum.

Caption: Endogenous EGFP reporter in green. Cell body expression in medial septum.

Terminal expression in the hippocampus.

Caption: Terminal expression in the hippocampus.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
3.00E+12 GC/ml
Injected Volume
8 μL- 1 injection
Injection Rate
0. 1 μl/min
Injection Site
Brain parenchyma
Brain Region
Medial septum
Injection Site Comments
We were injecting into medial septum and attempting to avoid lateral septum and diagonal band
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague-Dawley from Envigo
Age at Injection
Young adult (weight 300-325 grams)

Data Collection

Time After Injection
8 weeks for cell body expression, 12 weeks for terminal expression.
Detection Method
Immunohistochemistry
Expression Site
Viral expression was well-contained within the medial septum. Terminal expression was seen in common terminating regions and particularly widespread in the hippocampus
Genetic Tool Outcome
We injected CNO and vehicle when using this empty vector virus and the hM3Dq-containing virus and both worked as expected
Tips & Comments
0. 8 uL was perfect for effective expression within the medial septum without expanding too far into lateral and ventral regions. Leaving the needle in the region of infusion for a few minutes after the infusion is complete and raising the needle slowly is important for keeping the virus in the place of infusion