pAAV-hSyn-hM3D(Gq)-mCherry in Rat (AAV Retrograde)
Addgene #50474-AAVrg

This is the endogenous mCherry reporter showing good viral expression in the orbitofrontal cortex 12 weeks after infusion into the medial septum.

Caption: This is the endogenous mCherry reporter showing good viral expression in the orbitofrontal cortex 12 weeks after infusion into the medial septum.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
7.00E+12 GC/ml
Injected Volume
8 μL- 1 injection
Injection Rate
0. 1 μl/min
Injection Site
Brain parenchyma
Brain Region
Medial septum
Injection Site Comments
Non-specific cell type. We tried to inject exclusively into the medial septum and avoid lateral septum or the horizontal limb of the diagonal band
View in Addgene Catalog

Subject

Species / Strain
Rat Sprague-Dawley from Envigo
Age at Injection
Young adult (weight 300-325 grams)

Data Collection

Time After Injection
12 weeks
Detection Method
Immunohistochemistry
Expression Site
Expression was found throughout the brain in cell bodies of common afferents to the medial septum. These included the ventral tegmental area, lateral septum, and medial and orbital prefrontal cortices. The expression was not cell-type specific as far as we could tell
Genetic Tool Outcome
These studies were preliminary studies to determine viral location and spread. No CNO has been given yet so we do not know the answer to this question