pAAV.Syn.GCaMP6f.WPRE.SV40 in Mouse (AAV9)
Addgene #100837-AAV9

Green channel, Sutter MOM, Zeiss 20x 1.0NA. Image shows a maximum intensity projection across 200um in layer 2/3 neurons. In this animal, layer 4 neurons were also labeled, but they are deeper in the brain and are not shown in this image. This image is a maximum intensity projection from 100 microns down to roughly 300 microns. Layer 4 begins at about 400 microns deep.

Caption: Green channel, Sutter MOM, Zeiss 20x 1.0NA. Image shows a maximum intensity projection across 200um in layer 2/3 neurons. In this animal, layer 4 neurons were also labeled, but they are deeper in the brain and are not shown in this image. This image is a maximum intensity projection from 100 microns down to roughly 300 microns. Layer 4 begins at about 400 microns deep.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.28E+13 GC/ml
Injected Volume
4 sites, 200 nl per site
Injection Rate
0. 03 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Layer 2/3 cells
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
12 weeks

Data Collection

Time After Injection
2 weeks
Detection Method
Direct fluorescence, 2-photon microscopy, baseline fluorescence was clearly visible in layer 2/3, as well as spontaneous activity
Expression Site
Layer 2/3 neurons, and some Layer 4 cells
Other Information
At approximately 6 weeks, some cell nuclei were filled with GCaMP. The animal was then discarded
Tips & Comments
I would use a lower titer. Expression became visible already at 1 week, and GCaMP is known to be toxic expression is too high