pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 in Mouse (AAV1)
Addgene #98931-AAV1

Green channel, Sutter MOM, Zeiss 20x 1.0NA objective

Caption: Green channel, Sutter MOM, Zeiss 20x 1.0NA objective

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
7.67E+12 GC/ml
Injected Volume
2 injection sites, 200 nl each site
Injection Rate
0. 03 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Layer 5 neocortex
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
10 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Direct fluorescence
Expression Site
Layer 5 pyramidal neurons in S1 barrel field. This was an RPP4-cre mouse, which sparsely labels layer 5 pyramidal cells
Genetic Tool Outcome
Air puff stimuli were directed at the whisker, which evoked GluSnFR activity in layer 5 apical dendrites
Other Information
No toxicity or inflammation. The prep lasted over 3 months
Tips & Comments
No, this virus worked exactly as expected