pAAV.Syn.GCaMP6f.WPRE.SV40 in Mouse (AAV1)
Addgene #100837-AAV1

Green: GCaMP6f. Blue: DAPI. Microscope: Leica LSM 710 Confocal, 10X objective

Caption: Green: GCaMP6f. Blue: DAPI. Microscope: Leica LSM 710 Confocal, 10X objective

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.00E+12 GC/ml
Injected Volume
300 nl
Injection Rate
0. 015 μl/min
Injection Site
Brain parenchyma
Brain Region
Anterior insular cortex
Injection Site Comments
I used this virus to target neurons non-selectively in the anterior insular cortex and performed the photometry imaging of terminals in the nucleus accumbens
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Age at Injection
8-10 weeks

Data Collection

Time After Injection
5 weeks
Detection Method
Immunohistochemistry, In vivo imaging of photometry signals during behavior
Expression Site
Anterior insular cortex (with contamination in somatosensory cortex), without cell-type specificity
Other Information
More toxicity was observed after a very long time (more than 4 months) of expression
Tips & Comments
The optimal concentration of virus varies among brain regions. It is important to have a test of injecting various dilutions of virus in a specific brain region