AAV-hSyn1-GCaMP6s-P2A-nls-dTomato in Mouse (AAV Retrograde)
Addgene #51085-AAVrg

Co-localization of GCaMP6-derived GFP and tdTomato in ventral hippocampal CA1. Still shots taken with a confocal microscope.

Caption: Co-localization of GCaMP6-derived GFP and tdTomato in ventral hippocampal CA1. Still shots taken with a confocal microscope.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
7.69E+12 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Injected into prelimbic subregion of medial PFC. Targeting ventral hippocampal cells projecting to medial PFC
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
3 months

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence
Expression Site
Ventral hippocampal CA1 cells
Other Information
No toxicity/inflammation
Tips & Comments
Although GFP and tdTomato expression was seen in the ventral hippocampus, fluorescence was not dynamic - so didn't seem to work well for this cell class. We imaged in ex vivo hippocampal slices on a confocal microscope with a spinning disk