pENN.AAV.hSyn.Cre.WPRE.hGH in Mouse (AAV Retrograde)
Addgene #105553-AAVrg

White is AlexaFluor 647 secondary antibody fused to an anti-HA antibody. Cells are ventral hippocampal CA1 cells. Imaged on a confocal microscope at 20x magnification.

Caption: White is AlexaFluor 647 secondary antibody fused to an anti-HA antibody. Cells are ventral hippocampal CA1 cells. Imaged on a confocal microscope at 20x magnification.

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.20E+13 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Cerebral cortex (neocortex)
Injection Site Comments
Targeted the prelimbic subregion of the medial PFC
View in Addgene Catalog
Secondary Virus
AAV8.CAG.FLEX.BDNF:HA.WPRE
Injected Titer
4.20E+12 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Targeting ventral hippocampus for expression of BDNF in mPFC-projecting hippocampal neurons in CA1. Injected viruses during the same surgery

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
Mutant mice bred in-house (-e4 mice: Maynard et al., https://www.nature.com/articles/npp2015349)
Age at Injection
3 months

Data Collection

Time After Injection
6 weeks
Detection Method
Immunohistochemistry
Expression Site
Ventral CA1 pyramidal neurons
Genetic Tool Outcome
No tool
Other Information
No toxicity/inflammation
Tips & Comments
Viruses/titers used worked well