pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Rat (AAV8)
Addgene #44362-AAV8

Confocal (Zeiss LSM), 560nm channel, 20x objective

Caption: Confocal (Zeiss LSM), 560nm channel, 20x objective

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
2.50E+13 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Striatum (Putamen or caudate)
Injection Site Comments
Targeting nucleus accumbens
View in Addgene Catalog
Secondary Virus
CAV2-Cre
Injected Titer
2.50E+09 GC/ml
Injected Volume
500 nl
Injection Rate
100 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Basal Ganglia: Globus pallidus external
Injection Site Comments
Targeting ventral pallidum; injected 15 min following injection of AAV8-DIO-hM4Di

Subject

Species / Strain
Rat Wild-type Long Evans
Stock Number
Envigo rat stock no: 002
Age at Injection
6 weeks

Data Collection

Time After Injection
3 weeks
Detection Method
Immunohistochemistry
Expression Site
Indirect pathway medium spiny neurons of the nucleus accumbens
Genetic Tool Outcome
Yes - an injection of clozapine-N-oxide (ligand for receptor) but not vehicle had a significant behavioral effect
Other Information
No toxicity or inflammation. Healthy cellular morphology