pAAV-Syn-ChrimsonR-tdT in Mouse (AAV5)
Addgene #59171-AAV5

Chrimson-tdTomato in the basolateral amygdala

Caption: Chrimson-tdTomato in the basolateral amygdala

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
8.20E+12 GC/ml
Injected Volume
400 nl
Injection Rate
0. 1 μl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Amygdala
Injection Site Comments
Basolateral amygdala pyramidal cells, and their projections to the prefrontal cortex
View in Addgene Catalog
Secondary Virus
AAV-CaMKII-ChR2 was injected in a different site (MD thalamus) to examine convergent inputs in the prefrontal cortex.
Injected Titer
Unreported GC/ml
Injected Volume
400 nl
Injection Rate
0. 1 ul/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Thalamus
Injection Site Comments
Mediodorsal thalamus

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
Jax 000664
Age at Injection
4-6 weeks

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence, Confirmed red fluorescence in cell bodies in basolateral amygdala injection site and in axon terminals in prefrontal cortex. Then recorded excitatory postsynaptic currents in prefrontal cortex neurons evoked with light stimulation.
Expression Site
Basolateral amygdala projection neurons
Genetic Tool Outcome
Operated as expected
Other Information
Worked well
Tips & Comments
Worked as expected