pAAV-hSyn-DIO-hM4D(Gi)-mCherry in Mouse (AAV5)
Addgene #44362-AAV5

Ventral CA1, red is mCherry fused to hM4Di, imaged with LSM700 confocal, 20x objective lens. blue = DAPI.

Caption: Ventral CA1, red is mCherry fused to hM4Di, imaged with LSM700 confocal, 20x objective lens. blue = DAPI.

Virus & Injection

Primary Virus
Promoter
Syn
Injected Titer
4.20E+12 GC/ml
Injected Volume
600 nl
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Ventral hippocampus, targeting ventral CA1 Cre-expressing cells from injections of AAVrg-hSyn-EBFP-Cre in the mPFC
View in Addgene Catalog

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
3 months

Data Collection

Time After Injection
5 weeks
Detection Method
Direct fluorescence
Expression Site
Ventral CA1 neurons, presumably excitatory
Genetic Tool Outcome
No tool was used
Other Information
No toxicity, no inflammation
Tips & Comments
Expression of mCherry was pretty good. I might try a different serotype - hM4Di is tricky to express in subcortical cells