pAAV-hSyn-DIO-hM3D(Gq)-mCherry in Mouse (AAV8)
Addgene #44361-AAV8

blue = DAPI and red = mCherry, taken with a 20x objective on a confocal microscope. It's in the ventral dentate gyrus. Also see https://www.nature.com/articles/s41386-019-0429-1, Figure 2g

Caption: blue = DAPI and red = mCherry, taken with a 20x objective on a confocal microscope. It's in the ventral dentate gyrus. Also see https://www.nature.com/articles/s41386-019-0429-1, Figure 2g

Viruses & Injection

Primary Virus
Promoter
Syn
Injected Titer
1.00E+13 GC/ml
Injected Volume
two 300 nl injections
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Injected into ventral hippocampus - targeting Bdnf promoter IV-expressing cells. Expressed in dentate gyrus
View in Addgene Catalog
Secondary Virus
AAV8.p4BDNF.ERT2CreERT2.PEST
Injected Titer
2.00E+13 GC/ml
Injected Volume
2 x 300 nl injections
Injection Rate
200 nl/min
Injection Site
Brain parenchyma
Brain Region
Forebrain: Hippocampus
Injection Site Comments
Same as above - ventral hippocampus. This virus expresses Cre in promoter IV-expressing cells

Subject

Species / Strain
Mouse C57/BL6J
Stock Number
JAX: 000664
Age at Injection
3 months

Data Collection

Time After Injection
4 weeks
Detection Method
Direct fluorescence
Expression Site
Ventral dentate gyrus, putative excitatory neurons
Genetic Tool Outcome
Used 4OHT to express Cre, which facilitated expression of hM3Dq. MCherry. Worked as expected
Other Information
No toxicity, no inflammation, worked well
Tips & Comments
Used AAV8 to match serotype of other co-injected virus. Would not change anything